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首页> 《中国测试》期刊 >本期导读>快速识别水合肼荧光探针的合成及细胞成像研究

快速识别水合肼荧光探针的合成及细胞成像研究

173    2020-06-22

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作者:韩琪, 吴楠, 程思宇, 杨想, 包尚哲, 陈颂

作者单位:齐齐哈尔医学院药学院, 黑龙江 齐齐哈尔 161006


关键词:荧光探针;水合肼;分子内电荷转移;细胞成像


摘要:

该文以氰基联苯酚为荧光载体,乙酰基为识别基团,基于水合肼引发的脱保护反应和分子内电荷转移机制(ICT),研发一种能快速识别N2H4的开关型荧光探针。在pH=7.4(含1 mmol/L CTAB)的PBS缓冲溶液中,相对于其他测试的分子或离子,探针化合物对N2H4表现出良好的选择性和灵敏度,能在5 min内快速检测识别到N2H4。当探针浓度为10.0 μmol/L时,探针化合物对N2H4的检出限为0.08 μmol/L,这个限度符合美国环保局定义的N2H4浓度水平。在生理条件下,探针识别N2H4可以呈现显著的荧光开启信号。同时,细胞成像实验表明探针可以透过细胞膜与N2H4作用,为在线监测提供有效的工具分子。


Research on the synthesis and cell imaging of fast identifiable hydrazine fluorescent probe
HAN Qi, WU Nan, CHENG Siyu, YANG Xiang, BAO Shangzhe, CHEN Song
College of Pharmacy, Qiqihar Medical University, Qiqihar 161006, China
Abstract: Based on the deprotection reaction initiated by hydrazine and the mechanism of intramolecular charge transfer (ICT), a switch type fluorescent probe was developed to recognize N2H4 rapidly. Compared with other tested molecules and ions, the probe showed good selectivity and sensitivity to N2H4 in pH=7.4 PBS buffer solution containing 1 mmol/L CTAB, and it could detect N2H4 quickly within 5 min. When the concentration of the probe was 10.0 μmol/L, the detection limit of the probe to N2H4 was 0.08 μmol/L, which conforms to the concentration level of N2H4 defined by the U.S. Environmental Protection Agency. Under physiological conditions, the probe recognition of N2H4 showed a significant fluorescence signal. At the same time, the cell imaging experiments exhibited that the probe could react with N2H4 through the cell membrane, providing an effective tool molecule for on-line monitoring.
Keywords: fluorescent probe;hydrazine;intramolecular charge transfer;cell imaging
2020, 46(6):56-60  收稿日期: 2020-02-18;收到修改稿日期: 2020-03-21
基金项目: 黑龙江省大学生创新创业训练计划项目(201911230021)
作者简介: 韩琪(1997-),女,黑龙江大庆市人,专业方向为荧光探针的合成与生物学应用
参考文献
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